Device

Part:BBa_K190038:Design

Designed by: Nienke Kuipers   Group: iGEM09_Groningen   (2009-09-08)


Arsenic accumulation device (lactose promoter + fMT + GlpF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 246
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 335
    Illegal NgoMIV site found at 719
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The two genes were cloned in such a way that a polycistronic mRNA will be formed, but after translation two separate proteins will be produced as both still contain a stop-codon.


Source

The device is composed from BBa_K190032, which contains the lactose promoter (BBa_R0010) together with fMT ( BBa_K190019) , this is fused with BBa_K190028, GlpF the arsenic transporter.

References